Simultaneous spectrofluorimetric determination of glycerol and ethanol in wine by flow injection using immobilized enzymes
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چکیده
منابع مشابه
Simultaneous determination of D-glucose and 3-hydroxybutyrate by chemiluminescence detection with immobilized enzymes in a flow injection system.
A chemiluminometric flow-through sensor for the simultaneous determination of glucose (Glu) and 3-hydroxybutyrate (HB) in a single sample has been developed. Coimmobilized 3-hydroxybutyrate dehydrogenase/NADH oxidase/peroxidase, a support material, and coimmobilized glucose dehydrogenase/NADH oxidase/peroxidase were packed sequentially in a transparent PTFE tube. The tube was then placed in fro...
متن کاملDetermination of ethanol using flow injection enthalpimetry.
A flow injection enthalpimetric method for the determination of ethanol in fuel-grade ethanol, based on the heat of dilution of ethanol, is described. The accuracy and precision were 0.05% of ethanol; interferences by several substances are discussed. A similar procedure was also applied to the determination of ethanol in wines and spirits after distillation, yielding a precision of 0.0%0.1% of...
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A flow injection method was developed for the sequential enzymatic determination of ethanol and glycerol in wines, using immobilised ethanol dehydrogenase and glycerol dehydrogenase, respectively. The enzymes were immobilised separately on alkylaminated controlled pore glass. A multi-site spectrophotometric detection system was used in parallel configuration to monitor the absorbance change in ...
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Um procedimento relativamente simples e sensível com detecção espectrofluorimétrica foi desenvolvido para a determinação de cistina e cisteína por sistema de injeção em fluxo com determinação seqüencial. Esse método é fundamentado na redução de Tl(III) com cisteína em meio ácido, produzindo o reagente fluorescente TlCl 3 2(λ ex = 227 nm, λ em = 419 nm). Antes da injeção, a solução da amostra fo...
متن کاملSequential injection flow system with improved sample throughput: determination of glycerol and ethanol in wines
The performance of sequential injection (SI) systems has often been criticized for its low sampling frequency. The present work describes a SI system where an injection valve and an additional pump were incorporated to enhance sample throughput rate. The proposed system was applied to the enzymatic determination of glycerol and ethanol in wines, using spectrophotometric detection and immobilize...
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ژورنال
عنوان ژورنال: The Analyst
سال: 1995
ISSN: 0003-2654,1364-5528
DOI: 10.1039/an9952000179